Regulatory

Part:BBa_K1686098

Designed by: Nicolas Boisset   Group: iGEM15_Bordeaux   (2015-09-16)

His-Gal promoter

BBa_K1686098 is a biobrick containing a histidine auxotrophy gene of yeast and the promoter galactose one.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 21
    Illegal suffix found in sequence at 2048
    Illegal PstI site found at 1163
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 21
    Illegal SpeI site found at 2049
    Illegal PstI site found at 1163
    Illegal PstI site found at 2063
    Illegal NotI site found at 27
    Illegal NotI site found at 2056
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 21
    Illegal BglII site found at 1373
    Illegal BamHI site found at 2006
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 21
    Illegal suffix found in sequence at 2049
    Illegal PstI site found at 1163
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 21
    Illegal XbaI site found at 36
    Illegal SpeI site found at 2049
    Illegal PstI site found at 1163
    Illegal PstI site found at 2063
    Illegal AgeI site found at 1536
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 725

The sequence submitted in the part sequence is the initial sequence of the promoter. We have to do a mutagenesis to delete the different sites of restrictions that are not allowed in the final sequence. Our team had not the time to do it, that's why we only gave us the initial sequence in pSB1C3.


[edit]
Categories
//promoter
Parameters
None